This study investigates generational differences in leadership styles among healthcare professionals, focusing on Generation Z (Gen Z), Generation Y (Gen Y), and Generation X (Gen X). A cross-sectional survey was conducted with 122 participants from various healthcare institutions. Leadership preferences were assessed using a 15-item questionnaire measuring collaborative, adaptive, and supportive leadership characteristics on a 4-point Likert scale. Descriptive statistics and comparative analyses were employed to identify similarities and differences across generational cohorts. Findings indicate that all three generations strongly value collaborative and adaptive leadership, with Gen Y and Gen Z reporting higher preferences for flexibility, spokesperson roles, and inclusive decision-making than Gen X. By contrast, Gen X respondents scored lower on public-facing and developmental aspects, reflecting a more pragmatic, task-oriented approach. These results highlight the evolving nature of healthcare leadership, where younger professionals lean toward flexible and empathetic models, while older cohorts retain more traditional tendencies. The study contributes to understanding multigenerational dynamics in healthcare organizations and offers insights for leadership training and workforce development. Implications are discussed in relation to organizational performance, patient outcomes, and the United Nations Sustainable Development Goals (SDGs).