Background: Antibiotic resistance (ABR) is one of the main series global public health threats in the world. Its emergency among bacterial pathogens for local understand for the epidemiological situation. We needed information about that by gather both for clinical treatment decision-making purposes in addition to for the revision of present care guidelines. Objective: Our study was aimed to identify the local bacterial Uropathogens in south of Baghdad that appear antimicrobial resistance situation for major antibiotic in group of patients have history with Urinary tract infections (UTIs) Methodology: From February 2022 to October 2023, 51 patients which referred from hospital in south of Baghdad, Iraq with history of UTI. Five species of bacteria was isolated from samples which defined after culturing by microscopic, biochemical tests. Results: the study shown high significant differences at (P≤0.01) in bacterial infection in female compared with male, communal bacteria that reason UTI Escherichia coli (E.coli) appear high rates in female at 50%, while in male about 30.7%, as well as, staphylococcus aureus showed high rates after E.coli then other bacteria like Enterococcus sp., Klebsiella sp. E.coli showed high resistance against many antibiotic like Amikacin (AK), Amoxicillin-clavulanate (AUG), Azithromycin (AM), Cefotaxime (CTX), ceftriaxone (CRO) and other Beta-lactamase antibiotics, Gentamycin (CN), Levofloxacin (LEV), Tetracycline (TE) and Doxycycline (DXT). These isolates showed sensitive against Imipenem. Other results appeared most of bacteria like Klebsiella, Staphylococcus aureus and Pseudomonas sp. have resistance against Azithromycin and Gentamycin. Conclusion: Many studies performed to a dangerous about use antibiotic without any warnings that make most of UTIs pathogenic bacteria resistance especially E. coli for wide range of antibiotics that make us thinking thousands of times before using antibiotics without consult a doctor.